There are some challenges that one endures during the experiments. For instance, during transfer of bacteria one can easily contaminate the samples thus interfering with the results. Another challenge is that during the transfer of bacteria one could denature the cells due to the high temperatures of the inoculating wire during sterilization and hence interfering with the final result. Similarly, bacteria grown in room temperature will experience different temperatures this might interfere with the growth of bacteria, hence producing results that are not desired. Bacteria growth is affected by various factors for instance moisture oxygen and other factors and in the experiment variation of temperature is the factor to be studied hence if other factors are not constant then desired results will not be achieved.
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