Genetical diversity estimates for 12 populations of wood frogs were obtained in 12 separate island woodlots. The eggs were collected from the ponds and about thirty of them per pond, a maximum of 25 egg masses from each pond were sampled. The eggs from each egg mass were then placed in a separate plastic container with pond water and then transported to the laboratory where they were grown under controlled laboratory conditions. Three eggs from each egg mass were maintained in order to counteract possible problems that could occur during DNA extraction. The eggs were then subjected to Mylar, acetate and direct sunlight treatments. The UV-B measurements of the experimental room was about 25-285Âµ W/cm2Â and the Mylar filters blocked 90% of the UV-B while the acetate filters blocked about 36% of the UV-B.
Most mortality occurred at the larval stage and 186 tadpoles were deformed, 4 of them showed signs of edema, 181 had axial malformations and 1 had both axial malformation and edema. Exclusion of the sun treatment resulted in lowered mean lar
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